The presence of mosquitoes carrying Eastern Equine Encephalitis Virus (EEEV) on the rise in Connecticut, according to surveillance program from the Connecticut Agricultural Experiment Station and testing from UConn’s Connecticut Veterinary Medical Diagnostic Laboratory (CVMDL) in the College of Agriculture, Health and Natural Resources. Protect both humans and livestock with these steps: s.uconn.edu/eeev
Connecticut Veterinary Medical Diagnostic Laboratory
Avian Influenza in Connecticut
Songbird Alert

CVMDL (Drs. Mishra, Reinhardt, and Frasca) in UConn CAHNR are working with wildlife biologists of the CT DEEP to investigate this illness of songbirds; it is a disease concern throughout the mid-Atlantic and Northeast.
CVMDL – the Connecticut Veterinary Medical Diagnostic Laboratory – is coordinating sampling and testing with CT DEEP, who are working with wildlife rehabilitators.
We encourage you to take down your birdfeeders, follow DEEP’s cleaning recommendations, contact a licensed wildlife rehabilitator for guidance if you find a songbird in distress, and follow DEEP’s guidelines on disposal and reporting of dead songbirds.
More information can be found at s.uconn.edu/songbirds
Mystic Aquarium and CVMDL are Enhancing Animal Health and Learning Experiences
Visiting an aquarium transports people to another world, an underwater world filled with many different plants and animals. Mystic Aquarium in Mystic, Connecticut cares for 5,000 animals from over 355 species, from octopus to beluga whales and sea lions. Studying these animals offers a unique opportunity to learn about conserving these species in the wild, in support of Mystic Aquarium’s mission to care for and protect our ocean planet through conservation, education, and research.

Behind habitats that feature brightly colored fish and a diversity of other animals, like beluga whales that appear to engage with visitors, are the professionals – veterinarians, aquarists, trainers, divers, and environmental quality staff – that protect the health, safety, and wellbeing of the animals. The Connecticut Veterinary Medical Diagnostic Laboratory (CVMDL) in the Department of Pathobiology and Veterinary Science at UConn’s College of Agriculture, Health and Natural Resources is a partner in protecting and enhancing the health of the aquatic animals at Mystic Aquarium and has collaborated with its veterinarians for almost thirty years.
CVMDL partners with Mystic Aquarium on diagnostics and research to advance aquatic animal care and health and shares these initiatives with the larger scientific and educational communities.
The partnership between CVMDL and Mystic Aquarium started with Dr. Salvatore Frasca Jr., the Director of CVMDL. Dr. Frasca’s research focus is on pathology and diagnostics of aquatic animals. Service, through his appointment with UConn Extension and work with CVMDL, has always been a key component of his work.
“I chose UConn because it was close to Mystic Aquarium,” Dr. Frasca says. “I’m proud and pleased that CVMDL, through our service and educational activities, has been supporting Mystic Aquarium for over 30 years. We have a legacy of Extension activities at Mystic Aquarium that many people from both organizations have been part of.”
Dr. Frasca was enrolled in the Residency/PhD program in veterinary anatomic pathology at UConn in the 1990s after earning his veterinary degree from the University of Pennsylvania. Prior to that, he had worked closely with Dr. J. Lawrence Dunn, the veterinarian at Mystic Aquarium at the time. The two realized that there was a nationwide shortage of educational opportunities for veterinarians to learn aquatic animal medicine. Together, they wrote a grant to create an internship program at Mystic Aquarium.
Mystic Aquarium’s Internship in Aquatic Animal Medicine and Research teaches veterinarians how to apply and develop their skills for the benefit of aquatic animals and helps veterinarians further develop their expertise with research. It was the first aquatic marine animal veterinary internship ever offered, and Mystic Aquarium’s internship program has since trained nearly 30 veterinarians, many of whom are now leaders in the field of aquatic animal medicine, including two of the veterinarians currently employed at Mystic Aquarium. After receiving the grant, Dr. Frasca was encouraged to apply, and served as the first veterinary intern in the program in 1992. For nearly thirty years the program has continued to train veterinarians in aquatic animal health and has prompted similar opportunities in other locations.
The idea of developing educational experiences in clinical medicine and pathology was the foundation of the original partnership. CVMDL provides the pathology component and Mystic Aquarium has the clinical component. They are parallel tracks that weave back and forth because of the nature of the programs. Veterinarians come to UConn’s residency program in veterinary anatomic pathology, in part, because they want to experience aquatic animals. CVMDL integrates aquatic animals into the fabric of the training material and provides transformational learning experiences.
Enhancing Animal Health and Conservation

Dr. Allison Tuttle is the Senior Vice President of Zoologic Operations at Mystic Aquarium. She was introduced to Mystic Aquarium on a tour with the AQUAVET program, and then served as the Veterinary Intern in Aquatic Animal Medicine and Research from 2002 to 2004. Dr. Tuttle re-joined the aquarium as Director of Animal Care in 2007 when Dr. Dunn retired. In her current role, Dr. Tuttle oversees animal husbandry, animal rescue, veterinary services, environmental quality, the dive program, and exhibit interpretation.
“I fell in love with Mystic Aquarium on that initial tour,” Dr. Tuttle says. “There is a nice balance of opportunities among the programs we have, and we play a key role in wild marine animal rescue for our region.”
Mystic Aquarium’s Animal Rescue Program manages 1,000 miles of coastline and monitors deceased wild marine mammals and sea turtles to determine their cause of death. The aquarium sends samples to CVMDL for histopathologic analysis, which provides critically important information on what is happening in our oceans. The aquarium submits reports containing this data to federal agencies, and these reports are utilized to shape regulations and policies preventing fisheries and human interaction, which is important for marine mammal and sea turtle conservation.

“Our oceans are changing and there are a lot of unusual mortality events, in particular with large whale species in the greater Atlantic region,” Dr. Tuttle says. “As new issues emerge with the ocean it becomes increasingly important to monitor what is occurring. It’s tremendously difficult to study these whales; the data we collect is sometimes the only information we have on these animals.”
CVMDL’s pathology and diagnostic services have been integral to the health of Mystic Aquarium’s animals as well. They partner on sample analysis for their animals as well as on scientific collaborations. The aquarium sends samples to CVMDL for histopathologic analysis and biopsies on active clinical cases. The results from CVMDL guide care of the animals, important in the management of a healthy, robust population, by informing treatment and allowing for the best animal care possible.
Scientific research is another area where Mystic Aquarium and CVMDL collaborate. Aquatic clinical medicine and pathology go hand-in-hand. For example, Mystic Aquarium worked with CVMDL and the Connecticut Agricultural Experiment Station to report Eastern Equine Encephalitis (EEE) infection in a colony of penguins in 2003. EEE is an emerging health concern. Until that report, EEE infection in penguins had not been described. They published their findings in the Journal of the American Veterinary Medical Association.
“Mystic Aquarium has a great relationship with UConn and Dr. Frasca,” says Dr. Tuttle. “We have a strong connection with UConn and our conservation and animal care are greatly enhanced through our partnership with CVMDL.”
CVMDL is on the front lines of research and testing to keep humans and animals safe. The aquatic animal pathology service is one part of their work. Clients include those within the state, such as Mystic Aquarium and The Maritime Aquarium at Norwalk, and clients and aquariums from across the country. The laboratory offers diagnostic testing in support of pathology, including bacterial and fungal culture and molecular testing. The long-standing service history in aquatic animal pathology started with the relationship with Mystic Aquarium and now serves a number of other aquariums nationwide.
“CVMDL is committed to working with Mystic Aquarium, we’ve always known that Mystic Aquarium is a special asset for Connecticut,” Dr. Frasca concludes. “Extension activity is born of confluence and synergy of educational activities. That is exactly what happened with Mystic Aquarium.”
Article by Stacey Stearns
CVMDL Identifies Asian Longhorned Tick Submitted from NY

The Connecticut Veterinary Medical Diagnostic Laboratory (CVMDL) at UConn is reporting the identification of the Asian longhorned tick. This is the first time that CVMDL has identified this tick species. The ticks were submitted to CVMDL for identification and testing from the State of New York. Our laboratory notified New York State Animal and Public Health officials of the findings. This information was also reported to the USDA per regulations.
Ticks are disease-carrying arachnids that reside in moist areas, such as long grass and the leaf litter, and will latch onto humans and animals alike. Although there are many different species of ticks, people generally think of one tick species in particular when worrying about illness: the deer tick (Ixodes scapularis). While the Deer tick is predominantly known for transmitting the agent that causes Lyme disease (the corkscrew-shaped bacterium, Borrelia burgdorferi), it can also carry other disease-causing agents. A single tick can transmit more than one infectious agent.
Identification of the Asian longhorned tick at CVMDL is significant because it means their population is increasing and that presents another public health concern. Asian longhorned ticks are not traditionally found in the Western Hemisphere but were first identified here in 2017.
Although Asian longhorned ticks are not as attracted to humans as pets and livestock, the Centers for Disease Control and Prevention (CDC) and scientists at UConn’s CVMDL encourage people to take precautions against the tick. We are still uncertain of the diseases the Asian longhorned tick carries and spreads. The CDC offers guidelines to help people prevent tick bites.
CVMDL, part of the Department of Pathobiology in UConn’s College of Agriculture, Health and Natural Resources, is on the frontlines of research and testing to keep humans and animals safe. For more information visit http://cvmdl.uconn.edu or learn how to submit a tick test at https://bit.ly/HowToTestTick.
How do I get a tick tested?
Ticks carry many diseases that affect humans and animals. The Connecticut Veterinary Medical Diagnostic Laboratory in the Department of Pathobiology and Veterinary Science at UConn’s College of Agriculture, Health and Natural Resources offers tick testing. The steps to submit a tick are outlined in this video and you can visit https://bit.ly/UConnTickTesting to download the submission form.
Emergent Disease in Connecticut Deer Population Discovered
Connecticut Department of Energy and Environmental Protection (DEEP) and the Connecticut Veterinary Medical Diagnostic Laboratory (CVMDL) at UConn’s College of Agriculture, Health and Natural Resources (CAHNR) discovered an emergent disease in deer population in Connecticut.

In October of 2017 DEEP officials detected an unusual die-off of White Tail deer in central Connecticut. DEEP submitted carcasses to the Connecticut Veterinary Medical Diagnostic Laboratory (CVMDL) to establish possible causes of death. Necropsies were performed and tissues from the deer were analyzed by pathologists at the UConn laboratory. Anatomic changes observed in these tissues alerted pathologists to a disease never before recognized in Connecticut, “Epizootic Hemorrhagic Disease of deer” (EHD). Samples were referred to the Southeastern Cooperative Wildlife Disease Study at the University of Georgia where the presence of EHD virus (EHDV) was established.
EHDV causes a hemorrhagic disease in deer that is transmitted by midges, insects of the Culicoides spp. These insects also transmit the virus causing Bluetongue disease in domestic ruminants (goats, sheep and cattle). Bluetongue has not been found in Connecticut. There is a sustained expansion of these diseases in the United States linked to the geographical expansion of the transmitting vectors, in this case to northern latitudes.
DEEP and CVMDL have joined efforts over the years on discovering, detecting and reporting diseases affecting wildlife that, given environmental and ecological conditions, may spill over into livestock and human populations in the state of Connecticut.
This particular common effort, detecting EHDV in Connecticut, has initiated further studies at CVMDL to identify which species of Culicoides are responsible for transmission of the virus here in Connecticut. The Connecticut Agricultural Experiment Station (CAES) in New Haven is supporting this effort by providing CVMDL with Culicoides insects trapped across the State of Connecticut.
CVMDL, part of the Department of Pathobiology in UConn CAHNR, is on the frontlines of research and testing to keep humans and animals safe. For more information visit http://cvmdl.uconn.edu or call 860-486-3738.
Article written by CVMDL
Canine Circovirus in Connecticut, Identified by UConn Researchers

Investigators at the Connecticut Veterinary Medical Diagnostic Laboratory (CVMDL) at UConn recently reported a new canine disease, identified for the first time in New England. This is the same group, same laboratory, that recently reported eastern equine encephalomyelitis in horses and birds and earlier recognized epizootic hemorrhagic disease in deer (September 2017) and West Nile encephalitis in crows (2001).
The published case report (Journal of Veterinary Diagnostic Investigation, September 2019) documents the death of a 5-month-old dog that originated in Mississippi, was shelter-housed in Texas for a time, and then was delivered for adoption in Connecticut. The disease was characterized by severe bloody gastroenteritis and rapid progression to death. Autopsy was followed by electron microscopy and molecular techniques which demonstrated a circovirus as the cause of disease and death. First recognized in California in 2013, the appearance of canine circovirus disease in New England, in dogs shuttled among shelters, raises concerns for dog owners and veterinarians. At this time, it is hard to know if this disease will spread, like parvovirus disease in the 1980s, or remain sporadic.
CVMDL, part of the Department of Pathobiology in UConn’s College of Agriculture, Health and Natural Resources, is on the frontlines of research and testing to keep humans and animals safe. For more information visit http://cvmdl.uconn.edu or call 860-486-3738.
Article by Dr. H. J. Van Kruiningen
Information About EEE from CVMDL at UConn

CDC/James Gathany
Photographer: James Gathany
This is an Ochlerotatus triseriatus mosquito obtaining a blood meal from a human hand.
Also known as Aedes triseriatus, and commonly known as the ”treehole mosquito”, this species is a known West Nile Virus vector.
Photo by James Gathany, Centers for Disease Control
Eastern Equine Encephalitis (EEE) is a disease caused by a virus that mosquitos transmit. The name of the disease is misleading in that this virus can infect and cause disease in humans and a wide variety of animal species, including birds as well as horses and other equids. Horses that have not been vaccinated for EEE die within days of being infected as there is no treatment. There is an effective equine vaccine for EEE, however not for other species. Researchers and veterinarians UConn’s Connecticut Veterinary Medical Diagnostic Laboratory (CVMDL) encourage equine owners to consider vaccinating their animals, and other animal owners to implement measures to reduce mosquito habitats and thereby potential contact with mosquitos.
Mosquitos that feed on infected wild birds transmit EEE to horses and humans. Once infected, the virus attacks the central nervous system of the host. For horses, disease signs usually appear within five days and the clinical signs include fever, a dull or sleepy appearance, muscle twitches, and a weak staggering gait. Fatality in horses is 90% or higher as horses often go down and are unable to stand again, and those that do survive may have permanent brain damage.
EEE is transmitted by two main types of mosquito vectors; the primary vector and the bridging vector. Culiseta melanura, the primary vector which feeds almost exclusively on birds, serves to amplify and maintain the virus within wild bird populations. Other mosquito species, which indiscriminatingly feed on birds, horses, and humans, serve as the bridging vector capable of transmitting EEE from wildlife to horses and humans.
With the location of horse barns and pastures in rural areas the animals have increased exposure to mosquitos. Horses cannot pass EEE to humans, or to other horses, and are therefore referred to as a dead-end host. If an infected mosquito bites a human, that person can be infected and may develop disease. According to the Center for Disease Control, illness in humans due to EEE is rare, but when disease develops, it is serious.
Proactive steps can be taken to prevent EEE virus infection in humans and horses. A vaccine is available for horses, talk to your veterinarian about vaccinating annually for EEE. Mosquito control techniques include eliminating standing water, cleaning water troughs weekly, avoiding mosquito-infested areas, and using insect repellent.
CVMDL, part of the Department of Pathobiology in UConn’s College of Agriculture, Health and Natural Resources, is on the frontlines of research and testing to keep humans and animals safe. For more information visit http://cvmdl.uconn.edu or call 860-486-3738.
Reference
LSU Ag Center Research and Extension: http://www.lmca.us/PDF/pub2834eee.pdf
UConn CVMDL Tests Ticks for Disease-Causing Agents

Pet owners, livestock owners, and outdoor enthusiasts statewide need to maintain vigilance against ticks because as the fall season approaches will see an increase in their activity. UConn’s Connecticut Veterinary Medical Diagnostic Laboratory (CVMDL), part of the Department of Pathobiology & Veterinary Science in the College of Agriculture, Health and Natural Resources, is on the frontline of tick testing to inform submitters of the risks associated with that tick.
Ticks are disease-carrying arachnids that reside in moist areas, such as long grass and the leaf litter, and will latch onto humans and animals alike. Although there are many different species of ticks, people generally think of one tick species in particular when worrying about illness: the deer tick (Ixodes scapularis). While the Deer tick is predominantly known for transmitting the agent that causes Lyme disease (the corkscrew-shaped bacterium, Borrelia burgdorferi), it can also carry other disease-causing agents. A single tick can transmit more than one infectious agent.
“Our lab offers tick identification services, in addition to many other services,” says Dr. Joan Smyth, Director of CVMDL. Tick testing at CVMDL serves multiple purposes. It helps the person or veterinarian who submitted the tick understand the potential exposure of the subject that the tick was found on. Our researchers are also using the results from tick testing to track current and emerging disease producing agents carried by ticks, and to monitor for the spread of ticks that may have been recently introduced to our area, for example the long-horned tick. The data can be used in setting priority areas for prevention and vaccine development.
If you find a tick on yourself, your child, or your pet, remove it immediately. CVMDL can test the tick for pathogens. Ticks received at CVMDL are first examined under a microscope by trained technicians to determine the species of tick, life stage, and degree of blood engorgement, all of which are factors that may impact transmission of pathogens to the person or animal. Ticks may then be tested for the DNA of pathogens that are common to that tick species. Results are normally reported within three to five business days of receiving the sample, but next day testing is available for an additional fee.
Please send ticks together with a small square of moist paper towel, in sealed zip lock bags. The submission form, pricing and the “Do’s and don’ts of tick testing” can be found on our website at http://s.uconn.edu/468.
For more information, read the article from UConn Magazinethat includes tips to prevent tick bites, or watch the UConn Science in Seconds video. You can also contact the Connecticut Veterinary Medical Diagnostic Laboratory at cvmdl@uconn.edu or 860-486-3738 or visit the tick testing page on our website http://cvmdl.uconn.edu/service/tick.php.